Current aspects of qualitative and quantitative analysis of common yarrow (<i>Achillea Millefolium</i> L.)

Aim to develop a methodology for qualitative and quantitative analysis of flavonoids in the herb Achillea millefolium L.
 Material methods. The material study was yarrow harvested 2020 2021, an industrial sample from JSC "Krasnogorskleksredstva". Additionally, we used reference samples flavonoids: rutin, quercetin, kaempferol, cynaroside, luteolin, received previously during our studies raw materials containing described using NMR, UV mass spectroscopy. authenticity determined by thin layer chromatography (qualitative analysis). For total flavonoids, differential spectrophotometry, conducted accordance with Pharmacopoeial monograph 1.2.1.1.0003.15 "Spectrophotometry ultraviolet visible spectra". spectral characteristics water-ethanolic extractions were marked spectrophotometer "Specord 40" (Analytik Jena AG, Germany), cuvettes thickness 10 mm.
 Results. Based on results determination, revealed three adsorption zones bright yellow glow light at wavelength 365 nm (reagent AlCl3), one which level zone chromatogram solution standard cinaroside value Rf 0.7. dominant Rs 1.3 respect cynaroside presumably attributed cosmosiin, while 0.8 detected kaempferol. obtained data confirmed presence common yarrow, also possibility conduct given use as sample. We developed method determination spectrophotometry analytical wave length 400 nm. content varied 0.410.02% 0.740.03% (equivalent cynaroside). error single 95% confidence interval 6.70%.